HPLC Detectors - Types Comparison Principles {PDF PPT}*

 

hplc powerpoint presentation

May 22,  · HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - authorSTREAM Presentation. Types of HPLC: Types of HPLC Based on modes of chromatography: POLAR-POLAR = MORE AFFINITY NONPOLAR-NONPOLAR = MORE AFFINITY POLAR-NONPOLAR = LESS AFFINITY Normal Phase In this column type, the retention is governed by the interaction of the polar parts of the . Sep 22,  · This ppt gives basic ideas of HPLC instrumentation- authorSTREAM Presentation. Instrumentation of HPLC: PowerPoint Presentation: 9/22/ 37 Solvent Solvent strength e ° parameter, (adsorption) Solvent strength parameter, p ’ (partition) UV cut-off (nm) n -Hexane Cyclohexane Tetrachloromethane Apr 21,  · PPT: Chromatography PDF | PowerPoint Presentation: Few materials look homogenous but in real they are the combination of distinct substances.A very good example of such thing is the green plants as they have a mixture of distinct pigments.


HPLC INSTRUMENTATION |authorSTREAM


Copy embed code:, hplc powerpoint presentation. Automatically changes to Hplc powerpoint presentation or non-Flash embed. WordPress Embed Customize Embed. URL: Copy. Presentation Description No description available. By: charanrajtp 55 month s ago. Dear Madam, can you please send your presentation to my mail id charanrajtp gmail.

By: najeebshahul 58 month s ago. By: mithunsaha 64 month s ago. Pharm Ist year Raghu College of Pharmacy Introduction : Introduction Chromatography may be defined as a method of separating a mixture of component distribution component though equilibrium distribution between two phases.

This separation technique was introduced by J. Tswett in by Adsorption Chromatography. Firstly plant pigments are separated. All this occurs under pressure which may reach or exceed psi, hplc powerpoint presentation. What is HPLC?

The most widely used analytical separations technique Utilizes a liquid mobile phase to separate components of mixture Uses high pressure to push solvent through the column Popularity : sensitivity ready adaptability to accurate quantitative determination s uitability for separating nonvolatile species or thermally fragile ones HPLC is….

For retention to occur in normal phase, the packing must be more polar than the mobile phase with respect to the sample. Retention is the result of the interaction of the non-polar components of the solutes and the non-polar stationary phase.

Typical stationary phases are non-polar hydrocarbons, waxy liquids, or bonded hydrocarbons such as C18, C8, etc. Adsorption is a surface phenomenon.

Examples of HPLC stationary phases: Alumina Magnesium oxide Charcoal Cellulose Mobile Phase: Mobile Phase Mobile phase is the life line of HPLC system as it transports the sample from the injector to the detector and its characteristics such as composition, pH etc, have a profound effect on separation of sample components. The mobile phase should have the following desirable characteristics to carry out this important function. Affordable cost Non hazardous Inertness towards sample constituents and stationary phase Sample components should be hplc powerpoint presentation fully with the mobile phase Detector should not respond to mobile phase or to changes in the mobile phase composition as in gradient hplc powerpoint presentation Mobile phases hplc powerpoint presentation consist of water — organic solvent, aqueous buffer or mixtures of organic solvents with or without modifiers.

In isocratic mode the composition of the mobile phase remains unchanged throughout the analytical run whereas in the gradient elution mode mobile phase composition changes through programming of the pump. Porous particle packing, hplc powerpoint presentation. Dry packing technique : For relatively large particles, hplc powerpoint presentation. Slurry packing technique: For smaller particles. The packing used in modern HPLC consist of small, rigid particles size, hplc powerpoint presentation.

Again 3 types of column packing, 1. Porous,polymeric beds 2. Porous layer beds dia. Totally porous silica particles dia. Factors affecting column efficiency plate number : Factors affecting column efficiency plate number Column length Particle size Packing quality Linear velocity flow Instrument quality dead volume Hplc powerpoint presentation factor Band Broadening Van Deemeter equation : Band Broadening Hplc powerpoint presentation Deemeter equation Band broadening…: Band broadening… PowerPoint Presentation: A plate height equation expresses the correlation between plate height and mobile phase velocity.

Identical molecules travel differently in the column due to probability processes. The three processes that contribute to peak broadening described in the van Deemter equation are:: The three processes that contribute to peak broadening described in the van Deemter equation are: A-term: eddy diffusion: The column packing consists of particles with flow channels in between.

Due to the difference in packing and particle shape, hplc powerpoint presentation, the speed of the mobile phase in the various flow channels differs and analyte molecules travel along different flow paths through the channels, hplc powerpoint presentation. B-term: hplc powerpoint presentation diffusion: Molecules traverse the column under influence of the flowing mobile phase.

Due to molecular diffusion, slight dispersions of the mean flow rate will be the result. C-term: resistance against mass transfer: A chromatographic system is in dynamic equilibrium, hplc powerpoint presentation. As the mobile phase is moving continuously, the system has to restore this equilibrium continuously.

Since it takes some time to restore equilibrium resistance to mass transferthe concentration profiles of sample components between mobile and stationary phase are always slightly shifted. This results in additional peak broadening.

UV-visible absorption detector:: 1. Photodiode Array Detector: 2. Compatible with gradient elution, hplc powerpoint presentation. Disadvantages: Slightly less sensitive than UV-Visible detector. Schematic diagram of photodiode array detector 3. Refractive Index detector:: 3. Fluorescence detector:: 4. Fluorescence detector: For compounds having natural fluorescing capability Advantages extremely high sensitivity high selectivity Compatible with gradient elution Disadvantage may not yield linear response over wide range of concentrations PowerPoint Presentation: Fluorescence detector 5.

Evaporative Light Scattering Detector: 5. Electrochemical detectors: 6. Pump pressure - the higher in pressure, the higher in separation 3. Sample size 4. The polarity sample, solvent and column 5. Pharmaceuticals industry To control the drug stability Quantity of drug determination from pharmaceutical dosage forms, ex, hplc powerpoint presentation.

Paracetamol determination in panadol tablet Quantity of drug determination from biological fluids, ex: blood glucose level 2. Clinical test - Monitoring of hepatic chirosis patient through aquaporin 2 in the urine. Food and essence manufacture - sweetener analysis in the fruit juice - preservative analysis in sausage. However, the most significant changes have occurred in the column sorbent technology, detection, and automation.

HPLC has become the primary tool in the pharmaceutical industry in characterizing the purity and impurities of pharmaceutical products. In the next several years, advances in column technology particularly for the resolution of enantiomers and proteins will continueminimizing the all too common column-to-column variations.

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Edit Comment Close. Premium member. Presentation Transcript. Pharm Ist year Raghu College of Pharmacy. Introduction : Introduction Chromatography may be defined as a method of separating a mixture of component distribution component though equilibrium distribution between two phases.

PowerPoint Presentation: HPLC is an hplc powerpoint presentation process utilizing special instrument designed to separate, quailing and analyze components of Introduction a cervical armature. The most widely used analytical separations technique Utilizes a liquid mobile phase to separate components of mixture Uses high pressure to push solvent through the column Popularity : sensitivity ready adaptability to accurate quantitative determination s hplc powerpoint presentation for separating nonvolatile species or thermally fragile ones.

PowerPoint Presentation: Reverse Phase In this column the packing material is relatively non-polar and the solvent is polar with respect to the sample. PowerPoint Presentation: A pumping device generating a gradient of two different solvents, a steel enforced column and a detector for measuring the absorbance.

Stationary Phase: Stationary Phase The stationary phase is a solid. Mobile Phase: Mobile Phase Mobile phase is the life hplc powerpoint presentation of HPLC system as it transports the sample from the injector to the detector and its characteristics such as composition, pH etc, have a profound effect on separation of sample components. Types of column packing: Types of column packing Pellicular particle packing. Factors affecting column efficiency plate number : Factors affecting column efficiency plate number Column length Particle size Packing quality Linear velocity flow Instrument quality dead volume Retention factor.

PowerPoint Presentation: A plate height equation expresses the correlation between plate height and mobile phase velocity. Schematic diagram of hplc powerpoint presentation array detector. Refractive Index detector: Measure displacement of beam with respect to photosensitive surface of detector Advantages: respond to nearly all solutes reliable unaffected by flow rate low sensitive to dirt and air bubbles in the flow cell Disadvantages: expensive Low sensitivity cannot be used with gradient elution, hplc powerpoint presentation.

Fluorescence detector: For compounds having natural fluorescing capability Advantages extremely high sensitivity high selectivity Compatible with gradient elution Disadvantage may not yield linear response over wide range of concentrations. Evaporative Light Scattering Detector.

 

PPT: Chromatography | PDF | PowerPoint Presentation Free

 

hplc powerpoint presentation

 

Apr 21,  · PPT: Chromatography PDF | PowerPoint Presentation: Few materials look homogenous but in real they are the combination of distinct substances.A very good example of such thing is the green plants as they have a mixture of distinct pigments. Sep 29,  · OUTLINE• INTRODUCTION• TYPES OF CHROMATOGRAPHY• RP LIQUID CHROMATOGRAPHY• INSTRUMENTATION• CHROMATOGRAM• METHOD DEVELPOMENT LAYOUT• ADVANTAGES OF HPLC 3. INTRODUCTION • High performance liquid chromatography • A form of liquid chromatography used to separate compounds that are dissolved in solution. May 22,  · HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - authorSTREAM Presentation. Types of HPLC: Types of HPLC Based on modes of chromatography: POLAR-POLAR = MORE AFFINITY NONPOLAR-NONPOLAR = MORE AFFINITY POLAR-NONPOLAR = LESS AFFINITY Normal Phase In this column type, the retention is governed by the interaction of the polar parts of the .